Coding

Part:BBa_M36026

Designed by: Jon Rodriguez   Group: Stanford BIOE44 - S11   (2011-05-03)

phiX174 gene K - mutated rbs and start codon of C

Modified version of an optional PhiX174 gene that inhibits host cell DNA replication. Took the wt gene K from coliphage phiX174, and then mutated it synonymously in order to destroy the RBS and start codon of the overlapping gene C.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 112
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None